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KMID : 0383819610080020056
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Tuberculosis and Respiratory Diseases
1961 Volume.8 No. 2 p.56 ~ p.64
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Investigations in Tuberculin Production Part I : The amount of tuberculoprotein obtained on different media
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김현규(金賢奎)/Hyun Kyn Kim
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Abstract
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Glycerine broth and some symthetic media, including those co㎜only used for
tuberculin production, were compared in the following way. Three human starins were
inoculated on to the weight of the culture, the pH and the protein content of the media
was determined.
Larger crops of bacteria were obtained on the synthetic media to the glycerine broth.
Within the synthetic media differences were found as well in total growth as in the
growth rate of the bacteria.
Due to the natural content of proteins of glycerine broth, this medium could not
compared with the synthetic media with regard to the amount of protein formed. Among
the synthetic media Sauton medium had smaller protein content than the other media.
For the full grown cultures no simple relation was found between the total crop of
bacteria and the protein content of the culture filtrates. However, the cultures with a
low pH (pH=4-5) had a lower protein content than the filtrates with a higher final pH.
This may explain the small amount of protein obtained on Sauton medium, which had a
low final pH. Some irregularities in the growth curves for one of the strains are
supposed to be due to dissociation of the strain.
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